Relies on porous beads; larger molecules elute first because they are not trapped in small pores. Which chromatography method is this?

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Multiple Choice

Relies on porous beads; larger molecules elute first because they are not trapped in small pores. Which chromatography method is this?

Explanation:
Size Exclusion Chromatography uses porous beads as the stationary phase. Molecules separate by size because large molecules cannot penetrate many pores and travel a shorter, quicker path, so they elute first. Smaller molecules can enter the pores and take a longer, more tortuous route, causing them to elute later. This size-based separation is different from methods that rely on charge or specific binding, which are the primary principles behind ion exchange and affinity chromatography.

Size Exclusion Chromatography uses porous beads as the stationary phase. Molecules separate by size because large molecules cannot penetrate many pores and travel a shorter, quicker path, so they elute first. Smaller molecules can enter the pores and take a longer, more tortuous route, causing them to elute later. This size-based separation is different from methods that rely on charge or specific binding, which are the primary principles behind ion exchange and affinity chromatography.

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