First step of PCR; unravels DNA at about 95°C. What is this step called?

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Multiple Choice

First step of PCR; unravels DNA at about 95°C. What is this step called?

Explanation:
Denaturation is the step where the DNA double helix is heated to about 95°C to break the hydrogen bonds between base pairs, separating the two strands so each serves as a template in the next phase. This single-stranded template is then ready for primers to bind in the annealing step and for the polymerase to extend in the extension step. The other options describe different parts of the process: extension is when new DNA is synthesized, Taq polymerase is the enzyme used for synthesis rather than a step, and annealing is the primer-binding phase at a lower temperature.

Denaturation is the step where the DNA double helix is heated to about 95°C to break the hydrogen bonds between base pairs, separating the two strands so each serves as a template in the next phase. This single-stranded template is then ready for primers to bind in the annealing step and for the polymerase to extend in the extension step. The other options describe different parts of the process: extension is when new DNA is synthesized, Taq polymerase is the enzyme used for synthesis rather than a step, and annealing is the primer-binding phase at a lower temperature.

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