During PCR, which step involves primers binding to their complementary sequences on the DNA template?

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Multiple Choice

During PCR, which step involves primers binding to their complementary sequences on the DNA template?

Explanation:
Primers bind to complementary sequences during the annealing step. After the DNA is denatured into single strands, the reaction temperature is lowered so the short primers can hybridize specifically to their matching regions on the template. This binding provides the starting points for DNA polymerase to extend the new DNA strands in the following extension step. Denaturation separates strands; extension builds the new DNA; ligation isn’t part of standard PCR.

Primers bind to complementary sequences during the annealing step. After the DNA is denatured into single strands, the reaction temperature is lowered so the short primers can hybridize specifically to their matching regions on the template. This binding provides the starting points for DNA polymerase to extend the new DNA strands in the following extension step. Denaturation separates strands; extension builds the new DNA; ligation isn’t part of standard PCR.

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